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fruC [2018-11-23 17:17:34]
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fruC [2018-11-23 17:17:34]

fructokinase
Locus
BSU06170
Isoelectric point
4.62
Molecular weight
34.10 kDa
Protein length
320 aa Sequence Blast
Gene length
960 bp Sequence Blast
Function
utilization of sucrose and glucitol
Product
fructokinase
Essential
no
Synonyms
ydjE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
670,087 → 671,049

The protein

Catalyzed reaction/ biological activity

  • Fru ---→ Fru-6-P
  • Protein family

  • carbohydrate kinase pfkB family (according to Swiss-Prot)
  • Structure

  • 5EY7 (from Vibrio cholerae, 38% identity)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • GutR: activation, PubMed, in GutR regulon
  • Regulation

  • induced by glucitol (GutR) PubMed
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    Biological materials

    Mutant

  • MGNA-C213 (ydjE::erm), available at the NBRP B. subtilis, Japan
  • BKE06170 (ΔfruC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCACACTCATTTA, downstream forward: _UP4_TAAATCATTGAAGATGTTTC
  • BKK06170 (ΔfruC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCACACTCATTTA, downstream forward: _UP4_TAAATCATTGAAGATGTTTC