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fruC [2018-11-23 17:17:34]
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fruC [2018-11-23 17:17:34]

fructokinase
locus
BSU06170
pI
4.62
mw
34.10 kDa
protein length
320 aa Sequence Blast
gene length
960 bp Sequence Blast
function
utilization of sucrose and glucitol
product
fructokinase
essential
no
synonyms
ydjE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]

    • Gene

    Coordinates
    670,087 → 671,049

    The protein

    Catalyzed reaction/ biological activity

  • Fru ---→ Fru-6-P

    • Protein family

  • carbohydrate kinase pfkB family (according to Swiss-Prot)

    • Structure

  • [PDB|5EY7] (from Vibrio cholerae, 38% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]-[gene|B13F7C35FBD55C674E5B19836D32279E7C1A5520|gutP]-[gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8195086], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]: activation, [Pubmed|8195086], in [regulon|926BCA197F259558F72FDCC73998497B9B167D22|GutR regulon]

    • regulation

  • induced by glucitol ([protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]) [Pubmed|8195086]
    • view in new tab

    genes
    [gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-C213 (ydjE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2211 NBRP B. subtilis, Japan]
  • BKE06170 (Δ[gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCACACTCATTTA, downstream forward: _UP4_TAAATCATTGAAGATGTTTC
  • BKK06170 (Δ[gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCACACTCATTTA, downstream forward: _UP4_TAAATCATTGAAGATGTTTC